ARF5 Antibody
Rabbit Polyclonal
Catalog number: 15281-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | MCF7 cells, HeLa cells, human liver tissue, mouse brain tissue |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
MCF7 cells were subjected to SDS PAGE followed by western blot with 15281-1-AP(ARF5 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using 15281-1-AP(ARF5 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 15281-1-AP(ARF5 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ARF5 fusion protein ag7400 | Full name | ADP-ribosylation factor 5 |
|
Calculated molecular weight |
21kd |
Observed molecular
weight |
20kd |
|
GenBank accession number |
BC003043 | Gene ID (NCBI) | 381 |
| Gene symbol | ARF5 | Synonyms |
Background
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences. |
