Filter: AllIHCIF-P

IHC staining of mouse cerebellum using 17855-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 17855-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17855-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17855-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 17855-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using ARHGAP12 antibody (17855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat kidney using 17855-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat kidney tissue using ARHGAP12 antibody (17855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARHGAP12 Polyclonal antibody

Name: ARHGAP12 Polyclonal antibody
Brand: Proteintech
SKU: 17855-1-AP

ARHGAP12 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 17855-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

Rho GTPase-activating protein 12, Rho-type GTPase-activating protein 12

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in	human kidney tissue, mouse cerebellum tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue, rat kidney tissue

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17855-1-AP targets ARHGAP12 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12309 Product name: Recombinant human ARHGAP12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 486-841 aa of BC094719 Sequence: AVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKSSKKNVFELKTRQGTELLIQSDNDTVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDPKKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCIEHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMFFRELPEPLFTFNHFNDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMTYQSIAIVFGPTLLKPEKETGNIAVHTVYQNQIVELILLELSSIFGR Predict reactive species
Full Name	Rho GTPase activating protein 12
Calculated Molecular Weight	841 aa, 96 kDa
GenBank Accession Number	BC094719
Gene Symbol	ARHGAP12
Gene ID (NCBI)	94134
RRID	AB_3741937
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IWW6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP12 belongs to the Rho GTPase-activating protein (RhoGAP) family. RhoGAP is a key regulatory factor of the small GTPase Rho family and serves as a molecular switch that regulates various cellular processes. ARHGAP12 is involved in suppressing tumor formation by regulating cell invasion and adhesion.