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Alpha Sarcoglycan Monoclonal antibody

Alpha Sarcoglycan Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B10H7

Cat no : 67078-1-Ig

Synonyms

50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    WB analysis using 67078-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    WB analysis of human skeletal muscle using 67078-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    WB analysis of human heart using 67078-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    WB analysis using 67078-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of mouse skeletal muscle using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of mouse skeletal muscle using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of human liver using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of human liver using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of mouse heart using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of mouse heart using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of mouse tongue using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of mouse tongue using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of rat heart using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of rat heart using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of rat skeletal muscle using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IHC staining of rat skeletal muscle using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IF Staining of mouse heart using 67078-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IF Staining of mouse heart using 67078-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IF Staining of mouse skeletal muscle using 67078-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Alpha Sarcoglycan Monoclonal antibody (67078-1-Ig)

    IF Staining of mouse skeletal muscle using 67078-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human heart tissue, pig heart tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human liver tissue, mouse heart tissue, mouse tongue tissue, rat heart tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67078-1-Ig targets Alpha Sarcoglycan in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha Sarcoglycan fusion protein Ag28800
Full Name sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
Calculated Molecular Weight 387 aa, 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC025702
Gene Symbol SGCA
Gene ID (NCBI) 6442
RRIDAB_2882386
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for Alpha Sarcoglycan antibody 67078-1-IgDownload protocol
IHC protocol for Alpha Sarcoglycan antibody 67078-1-IgDownload protocol
IF protocol for Alpha Sarcoglycan antibody 67078-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols