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BMP6 Polyclonal antibody

BMP6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55421-1-AP

Synonyms

BMP 6, BMP6, BMP-6, bone morphogenetic protein 6, VG 1 R, VG 1 related protein, VGR, VGR 1, VGR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BMP6 Polyclonal antibody (55421-1-AP)

    WB analysis using 55421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using BMP6 Polyclonal antibody (55421-1-AP)

    WB analysis of mouse liver using 55421-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BMP6 Polyclonal antibody (55421-1-AP)

    IHC staining of human tonsillitis using 55421-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BMP6 Polyclonal antibody (55421-1-AP)

    IHC staining of human tonsillitis using 55421-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using BMP6 Polyclonal antibody (55421-1-AP)

    IHC staining of human liver using 55421-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using BMP6 Polyclonal antibody (55421-1-AP)

    IHC staining of human liver using 55421-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, mouse liver tissue, HEK-293 cells, HL-60 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55421-1-AP targets BMP6 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name bone morphogenetic protein 6
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberNM_001718
Gene Symbol BMP6
Gene ID (NCBI) 654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bone morphogenetic protein 6 (BMP6) is also named as VG-1-related protein (VG-1-R, VGR-1) and VGR, and belongs to the TGF-beta family. (PMID:33878365). Growth factor of the TGF-beta superfamily plays essential roles in many developmental processes including cartilage and bone formation (PMID:31019025). BMP6 participates in iron homeostasis, inhibits invasion by increasing adhesions and cell-cell type interactions and induces angiogenesis directly on vascular endothelial cells. BMP6 is coded by a tumor suppressor gene whose subexpression is related to the development and cancer progression; during neoplastic processes, methylation is the main mechanism by which gene silencing occurs (PMID:34706618). BMP6 plays an important role in the regulation of hepcidin/HAMP expression and iron metabolism by acting as a ligand for hemojuvelin/HJV (PMID:26582087). BMP6 initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B (PMID:18070108).

Protocols

Product Specific Protocols
WB protocol for BMP6 antibody 55421-1-APDownload protocol
IHC protocol for BMP6 antibody 55421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols