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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 2 publications below |
Product Information
55417-1-AP targets CASPR in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | contactin associated protein 1 |
| Calculated Molecular Weight | 156 kDa |
| Observed Molecular Weight | 180-190 kDa |
| GenBank Accession Number | NM_003632 |
| Gene Symbol | CASPR |
| Gene ID (NCBI) | 8506 |
| RRID | AB_11232211 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78357 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CNTNAP1, also named as CASPR, NRXN4, p190, Neurexin IV and Neurexin-4, belongs to the neurexin family. It plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. CNTNAP1 seems to demarcate the paranodal region of the axo-glial junction. In association with contactin, CNTNAP1 may have a role in the signaling between axons and myelinating glial cells. This antibody is specific to CNTNAP1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CASPR antibody 55417-1-AP | Download protocol |
| IHC protocol for CASPR antibody 55417-1-AP | Download protocol |
| WB protocol for CASPR antibody 55417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Inhibition of SLC11A1-Mediated Lysosomal Iron Accumulation in Microglia Promotes Repair Following White Matter Stroke. | ||
Front Psychiatry Analysis of the influences of social isolation on cognition and the therapeutic potential of deep brain stimulation in a mouse model |
