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CHAC1 Polyclonal antibody

CHAC1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15207-1-AP

Synonyms

Blocks Notch protein, Botch, CHAC1



Tested Applications

Positive WB detected inHeLa cells, A549 cells, CHO cells, U-251 cells, C6 cells, RAW 264.7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15207-1-AP targets CHAC1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity human, mouse, rat, hamster
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHAC1 fusion protein Ag7360
Full Name ChaC, cation transport regulator homolog 1 (E. coli)
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC001847
Gene Symbol CHAC1
Gene ID (NCBI) 79094
RRIDAB_2878118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHAC1(Cation transport regulator-like protein 1) was identified in a co-regulated group of genes enriched for components of the ATF4 (activating transcription factor 4) arm of the unfolded protein response pathway. CHAC1 is a proapoptotic ER stress protein downstream of the pancreatic EIF2α kinase-ATF4 pathway that appears to be important for human physiology and disease(PMID: 19109178). In addition, the study found that high CHAC1 expression is associated with a bad prognosis hinting that CHAC1 may have a possible prognostic significance in breast cancer(PMID: 35930144). The predicted molecular weight of CHAC1 is 24 kDa, and the molecular weight detected by Proteintech is 38 kDa.

Protocols

Product Specific Protocols
WB protocol for CHAC1 antibody 15207-1-APDownload protocol
IHC protocol for CHAC1 antibody 15207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Proc Natl Acad Sci U S A

Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts

Authors - Adelyn A Crabtree
mouseWB

Cell Mol Gastroenterol Hepatol

Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis.

Authors - Sha Huang
humanWB

Free Radic Biol Med

High-fat diet aggravates colitis-associated carcinogenesis by evading ferroptosis in the ER stress-mediated pathway.

Authors - Xiaoli Zhang
humanWB

Lung

ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis

Authors - Min Yang
humanWB

Front Oncol

Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer

Authors - Pengyu Fan
humanIHC

J Cell Mol Med

Ferroptosis-related gene NOX4, CHAC1 and HIF1A are valid biomarkers for stomach adenocarcinoma.

Authors - Ruoxi Xiao