CIITA Polyclonal antibody

CIITA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55099-1-AP

Synonyms

C2TA, CIITA, CIITAIV, MHC class II transactivator, MHC2TA, NLRA



Tested Applications

Positive WB detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55099-1-AP targets CIITA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name class II, major histocompatibility complex, transactivator
Calculated Molecular Weight 124 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberNM_000246
Gene Symbol CIITA
Gene ID (NCBI) 4261
RRIDAB_2881268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIITA, also naned as MHC2TA, is a 1130 amino acid protein, which Interacts with ZXDA and ZXDC. MHC class II molecules are heterodimeric cell surface glycoproteins expressed on B cells, macrophages and dendritic cells, which present antigens to CD4+ T cells. CIITA (class II transactivator) acts as a coactivator for MHC class II-specific gene expression and negatively regulates the IL-4 gene promoter during T cell differentiation. IFNg induces CIITA gene expression via JAK1 and Stat1 pathways. T Defects of CIITA and RFX-B have been implicated as causes of Bare Lymphocyte Syndrome (BLS), which is characterized by the absence of MHC class II transcription and severe immunodeficiencies.

Protocols

Product Specific Protocols
WB protocol for CIITA antibody 55099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Identification and validation of m6A-GPI signatures as a novel prognostic model for colorectal cancer

Authors - Bin Ma