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CWC22 Polyclonal antibody

CWC22 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 26898-1-AP

Synonyms

CWC22, EIF4GL, fSAPb, KIAA1604, NCM, Nucampholin homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CWC22 Polyclonal antibody (26898-1-AP)

    WB analysis of A549 using 26898-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26898-1-AP (CWC22 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CWC22 Polyclonal antibody (26898-1-AP)

    WB analysis of HepG2 using 26898-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26898-1-AP (CWC22 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26898-1-AP is CWC22 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CWC22 fusion protein Ag25466
Full Name CWC22 spliceosome-associated protein homolog (S. cerevisiae)
Calculated Molecular Weight 908 aa, 106 kDa
Observed Molecular Weight 105-110 kDa
GenBank Accession NumberBC093954
Gene Symbol CWC22
Gene ID (NCBI) 57703
RRIDAB_2880674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CWC22, Pre-mRNA-splicing factor CWC22 homolog, is a 908 amino acid protein, which contains 1 MI domain and 1 MIF4G domain and belongs to the CWC22 family. CWC22 is required for pre-mRNA splicing and for exon-junction complex (EJC) assembly and hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs.

Protocols

Product Specific Protocols
WB protocol for CWC22 antibody 26898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation.

Authors - Cole Townsend
View Article