EIF5A Antibody
Rabbit Polyclonal
Catalog number: 11309-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells |
| Recommended dilution | WB: 1:200-1:2000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 11309-1-AP(EIF5A Antibody) at dilution of 1:800
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | EIF5A fusion protein ag1849 | Full name | eukaryotic translation initiation factor 5A |
|
Calculated molecular weight |
18kd |
Observed molecular
weight |
18kd |
|
GenBank accession number |
BC001832 | Gene ID (NCBI) | 1984 |
| Gene symbol | EIF5A | Synonyms | EIF-5A; EIF5A1; MGC104255; MGC99547; eIF5AI |
Background
Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation. |
