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  • KD/KO Validated

ETS1 Monoclonal antibody

ETS1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4F2D4

Cat no : 66598-1-Ig

Synonyms

ETS 1, ETS1, EWSR2, p54, Protein C ets 1



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, Daudi cells, HSCT6 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66598-1-Ig targets ETS1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ETS1 fusion protein Ag2127
Full Name v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Calculated Molecular Weight 441 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC017314
Gene Symbol ETS1
Gene ID (NCBI) 2113
RRIDAB_2881958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.

Protocols

Product Specific Protocols
WB protocol for ETS1 antibody 66598-1-IgDownload protocol
IF protocol for ETS1 antibody 66598-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Med

ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.

Authors - Lihong Lu
  • KD Validated
humanWB

Blood Adv

ETS1 is a novel transcriptional regulator of adult T-cell leukemia/lymphoma of North American descent

Authors - Rebecca A Luchtel