HDAC3-specific Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HL-60 cells, human stomach tissue, U-937 cells |
| Recommended dilution | WB: 1:200-1:2000 |
IP = Immunoprecipitation
HL-60 cells were subjected to SDS PAGE followed by western blot with 16164-1-AP(HDAC3-specific Antibody) at dilution of 1:500
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | Peptide | Full name | histone deacetylase 3 |
|
Calculated molecular weight |
49kd |
Observed molecular
weight |
50kd |
|
GenBank accession number |
NM_003883 | Gene ID (NCBI) | 8841 |
| Gene symbol | HDAC3 | Synonyms | HD3; RPD3; RPD3-2 |
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC3. This antibody is specific to HDAC3, and will not cross react with other HDACs. |
