Human IL-10 ELISA Kit

Sensitivity

0.5 pg/mL

Range

7.8-500 pg/mL

Reactivity

Human

Cat no : KE00012

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Product Information

KE00012 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human IL-10 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.5 pg/mL
Range 7.8-500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3586

Recovery

Sample TypeAverageRange
Human plamsa100%80%-127%
Cell culture supernatants101%87%-114%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12038.91.74.3
220133.47.05.2
320484.917.13.5

InterAssay

Samplenmean ( pg/mL)SDCV%
12439.92.46.1
224137.57.85.7
32452739.77.5

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Proc Natl Acad Sci U S A

miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression.

Authors - Chengxin Ma
humancell culture supernatants

Cancer Lett

MiR-26b-5p in small extracellular vesicles derived from dying tumor cells after irradiation enhances the metastasis promoting microenvironment in esophageal squamous cell carcinoma.

Authors - Xiaoyang Yin
humancell culture supernatants

Oncogene

Hypoxic glioma-derived exosomes deliver microRNA-1246 to induce M2 macrophage polarization by targeting TERF2IP via the STAT3 and NF-κB pathways.

Authors - Mingyu Qian
plasma

J Nutr Biochem

Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats.

Authors - Priscila Haygert
humancell culture supernatants

Oncogenesis

Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer.

Authors - Yaping Lv
humanserum

Antibiotics (Basel)

Rifaximin Alleviates Endotoxemia with Decreased Serum Levels of Soluble CD163 and Mannose Receptor and Partial Modification of Gut Microbiota in Cirrhotic Patients.

Authors - Kosuke Kaji