Human NINJ2 ELISA Kit
Sensitivity
0.2 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Cat no : KE00038
Synonyms
Nerve injury induced protein 2, NINJ2, ninjurin 2
Validation Data Gallery
Product Information
KE00038 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NINJ2 ELISA kit is to be used to detect and quantify protein levels of endogenous NINJ2. The assay recognizes human NINJ2. A polyclonal antibody specific for NINJ2 has been pre-coated onto the microwells. The NINJ2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for NINJ2 is added to detect the captured NINJ2 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
Product name | Human NINJ2 ELISA Kit |
Tests | 1 X 96 well plate |
Sample type | Plasma, Serum |
Assay type | Sandwich |
Sensitivity | 0.2 ng/mL |
Range | 0.313-20 ng/mL |
Reactivity | Human |
Tested applications | Sandwich ELISA |
Gene ID (NCBI) | 4815 |
Recovery
Sample Type | Average | Range |
---|---|---|
Plasma | 86% | 72%-102% |
IntraAssay
Sample | n | mean ( ng/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | ? ug/ml | ? | ?% |
2 | 20 | ? ug/ml | ? | ?% |
3 | 20 | ? ug/ml | ? | ?% |
InterAssay
Sample | n | mean ( ng/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | ? ug/ml | ? | ?% |
2 | 20 | ? ug/ml | ? | ?% |
3 | 20 | ? ug/ml | ? | ?% |
Background Information
NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. NINJ2 gene polymorphism is associated with stroke risk, and an association has also been reported between NINJ2 gene polymorphism and Alzheimer's disease risk.
Properties
Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Synonyms | Nerve injury induced protein 2, NINJ2, ninjurin 2 |