• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

KDM3B Polyclonal antibody

KDM3B Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19915-1-AP

Synonyms

5qNCA, C5orf7, JHDM2B, JMJD1B, KDM3B, KIAA1082, lysine (K) demethylase 3B, Lysine specific demethylase 3B, NET22, Nuclear protein 5qNCA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using KDM3B Polyclonal antibody (19915-1-AP)

    WB analysis using 19915-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using KDM3B Polyclonal antibody (19915-1-AP)

    WB analysis of human placenta using 19915-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using KDM3B Polyclonal antibody (19915-1-AP)

    IF Staining of A431 using 19915-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KDM3B antibody (19915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, HeLa cells, HepG2 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19915-1-AP targets KDM3B in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDM3B fusion protein Ag13795
Full Name lysine (K)-specific demethylase 3B
Calculated Molecular Weight 1761 aa, 192 kDa
Observed Molecular Weight 192 kDa, 85 kDa
GenBank Accession NumberBC001202
Gene Symbol KDM3B
Gene ID (NCBI) 51780
RRIDAB_10693529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KDM3B antibody 19915-1-APDownload protocol
IF protocol for KDM3B antibody 19915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
View Article