KHDRBS1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IHC |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:100 |
IP = Immunoprecipitation
HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP(KHDRBS1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using 10222-1-AP(KHDRBS1 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using KHDRBS1 antibody 10222-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | KHDRBS1 fusion protein ag0235 | Full name | KH domain containing, RNA binding, signal transduction associated 1 |
|
Calculated molecular weight |
48kd |
Observed molecular
weight |
68kd |
|
GenBank accession number |
BC000717 | Gene ID (NCBI) | 10657 |
| Gene symbol | KHDRBS1 | Synonyms | FLJ34027; Sam68; p62 |
KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human KHDRBS1. |
