LENG9 Polyclonal antibody

LENG9 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16295-1-AP

Synonyms

LENG9



Tested Applications

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human heart tissue, human liver tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16295-1-AP targets LENG9 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LENG9 fusion protein Ag9481
Full Name leukocyte receptor cluster (LRC) member 9
Calculated Molecular Weight 501 aa, 53 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC015921
Gene Symbol LENG9
Gene ID (NCBI) 94059
RRIDAB_2296934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LENG9 antibody 16295-1-APDownload protocol
IHC protocol for LENG9 antibody 16295-1-APDownload protocol
IF protocol for LENG9 antibody 16295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Long-read sequencing unveils high-resolution HPV integration and its oncogenic progression in cervical cancer.

Authors - Liyuan Zhou