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EDG2 Polyclonal antibody

EDG2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20442-1-AP

Synonyms

edg 2, EDG2, Gpcr26, GPR26, LPA 1, LPA receptor 1, LPA1, LPAR1, Mrec1.3, rec.1.3, vzg 1, VZG1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using EDG2 Polyclonal antibody (20442-1-AP)

    WB analysis of Jurkat using 20442-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of multi-cells/tissue using EDG2 Polyclonal antibody (20442-1-AP)

    WB analysis of multi-cells/tissue using 20442-1-AP

    Multi-cells/tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EDG2 Polyclonal antibody (20442-1-AP)

    WB analysis of HeLa using 20442-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using EDG2 Polyclonal antibody (20442-1-AP)

    WB analysis of A375 using 20442-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using EDG2 Polyclonal antibody (20442-1-AP)

    WB analysis of mouse brain using 20442-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EDG2 Polyclonal antibody (20442-1-AP)

    IHC staining of mouse brain using 20442-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EDG2 Polyclonal antibody (20442-1-AP)

    IHC staining of mouse brain using 20442-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using EDG2 Polyclonal antibody (20442-1-AP)

    IHC staining of human brain using 20442-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using EDG2 Polyclonal antibody (20442-1-AP)

    IHC staining of human brain using 20442-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, A375 cells, HeLa cells, mouse brain tissue, multi-cells/tissue, A549 cells, A2780 cells, NIH3T3 cells
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20442-1-AP targets EDG2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDG2 fusion protein Ag14253
Full Name lysophosphatidic acid receptor 1
Calculated Molecular Weight 364 aa, 41 kDa
Observed Molecular Weight 41-50 kDa
GenBank Accession NumberBC030615
Gene Symbol LPAR1
Gene ID (NCBI) 1902
RRIDAB_11183048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDG2 (also known as LPA1) is a G-protein coupled receptor for lysophosphatidic acid (LPA), a potent motility inducing factor, which is a major component of serum (PMID: 19415462). EDG2 is widely expressed in normal tissue during growth and development. In the context of cancer, several studies have suggested that EDG2 expression in tumors is often similar to that shown in normal tissue (PMID: 17496233).

Protocols

Product Specific Protocols
WB protocol for EDG2 antibody 20442-1-APDownload protocol
IHC protocol for EDG2 antibody 20442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mediators Inflamm

AM966, an Antagonist of Lysophosphatidic Acid Receptor 1, Increases Lung Microvascular Endothelial Permeability through Activation of Rho Signaling Pathway and Phosphorylation of VE-Cadherin.

Authors - Junting Cai
View Article