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  • KD/KO Validated

LSM12 Monoclonal antibody

LSM12 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E3G3

Cat no : 67029-1-Ig

Synonyms

FLJ30656, LSM12, LSM12 homolog (S. cerevisiae), PNAS 135, Protein LSM12 homolog



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67029-1-Ig targets LSM12 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LSM12 fusion protein Ag28782
Full Name LSM12 homolog (S. cerevisiae)
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC044587
Gene Symbol LSM12
Gene ID (NCBI) 124801
RRIDAB_2882344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LSM12 antibody 67029-1-IgDownload protocol
IHC protocol for LSM12 antibody 67029-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Signal

Convergent activation of Ca2+ permeability in two-pore channel 2 through distinct molecular routes

Authors - Ryo Saito
  • KO Validated