Mcl-1l-specific Antibody
Mouse Monoclonal
Catalog number: 65025-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | BCBL-1 cells |
| Positive IHC detected in | Lymphoma |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
BCBL-1 cells were subjected to SDS PAGE followed by western blot with 65025-1-Ig(Mcl-1l-specific Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded Lymphoma using 65025-1-Ig(Mcl-1l-specific Antibody) at Dilution 1:100
Product Information
| Source | Mouse | Purification method | Protein G purification |
| Isotype | IgG1 | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Recombinant Protein | Full name | myeloid cell leukemia sequence 1 (BCL2-related) |
|
Calculated molecular weight |
37kd |
Observed molecular
weight |
|
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GenBank accession number |
NM_021960 | Gene ID (NCBI) | 4170 |
| Gene symbol | MCL1 | Synonyms | BCL2L3; EAT; MCL1L; MCL1S; MGC104264; MGC1839; Mcl-1; TM |
Background
MCL1 is a potent multidomain antiapoptotic protein belonging to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1, 37-40kd) enhances cell survival by inhibiting apoptosis, while the alternatively spliced shorter gene product (isoform 2, 29-32kd) promotes apoptosis and is death-inducing. This antibody is specific to long isoform of MCL protein. |
