PCNA Antibody
Mouse Monoclonal
Catalog number: 65034-1-Ig
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | 293 cells |
| Recommended dilution | WB: 1:500-1:5000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
293 cells were subjected to SDS PAGE followed by western blot with 65034-1-Ig(PCNA Antibody) at dilution of 1:1000
Product Information
| Source | Mouse | Purification method | Caprylic Acid/Ammonium Sulfate Precipitation |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Recombinant Protein | Full name | proliferating cell nuclear antigen |
|
Calculated molecular weight |
29kd/31kd |
Observed molecular
weight |
36kd |
|
GenBank accession number |
BC000491 | Gene ID (NCBI) | 5111 |
| Gene symbol | PCNA | Synonyms | MGC8367 |
Background
The proliferating cell nuclear antigen (PCNA) is a 36 kDa protein and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is synthesized in early G1 and S phases of the cell cycle and serve as an excellent marker for proliferating cells. In early S phase, PCNA exhibits a very granular distribution and is absent from the nucleus, while in late S phase, it is prominent in the nucleoli. |
