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PI3 Kinase p55 Gamma Polyclonal antibody

PI3 Kinase p55 Gamma Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27035-1-AP

Synonyms

p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3



Tested Applications

Positive WB detected inMCF-7 cells, HUVEC cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27035-1-AP targets PI3 Kinase p55 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI3 Kinase p55 Gamma fusion protein Ag25884
Full Name phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC021622
Gene Symbol PIK3R3
Gene ID (NCBI) 8503
RRIDAB_2880730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p55 Gamma antibody 27035-1-APDownload protocol
IHC protocol for PI3 Kinase p55 Gamma antibody 27035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Physiol Lung Cell Mol Physiol

p55PIK Deficiency and its N-terminal Derivative inhibit Inflammation and Emphysema in COPD mouse model.

Authors - Ting Wang
human,mouseWB,IHC

BMC Cancer

Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma

Authors - Cheng Tan
  • KD Validated