PSMB11 Polyclonal antibody

PSMB11 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 55143-1-AP

Synonyms

beta5t, Proteasome subunit beta 5t, PSMB11



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55143-1-AP targets PSMB11 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name proteasome (prosome, macropain) subunit, beta type, 11
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberNM_001099780
Gene Symbol PSMB11
Gene ID (NCBI) 122706
RRIDAB_10863288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.

Protocols

Product Specific Protocols
WB protocol for PSMB11 antibody 55143-1-APDownload protocol
IHC protocol for PSMB11 antibody 55143-1-APDownload protocol
FC protocol for PSMB11 antibody 55143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anja (Verified Customer) (03-16-2022)

We tested the antibody in different dilutions on different cells of the thymus and Psmb11 knockout (KO) mice and could not obtain a specific staining with the antibody. In our hands the signal in the Psmb11-KO mice was comparable to the one obtained from either thymocytes or thymus epithelial cells of Wildtype mice .

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Thymus