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  • Featured Product
  • KD/KO Validated

RAB3GAP1 Monoclonal antibody

RAB3GAP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4A3A1

Cat no : 66688-1-Ig

Synonyms

DKFZp434A012, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    WB analysis using 66688-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    WB analysis of HeLa using 66688-1-Ig

    WB result of RAB3GAP1 antibody (66688-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of mouse brain using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of mouse brain using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of human testis using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IHC staining of human testis using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RAB3GAP1 Monoclonal antibody (66688-1-Ig)

    IF Staining of HEK-293 using 66688-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAB3GAP1 antibody (66688-1-Ig, Clone: 4A3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66688-1-Ig targets RAB3GAP1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB3GAP1 fusion protein Ag17710
Full Name RAB3 GTPase activating protein subunit 1 (catalytic)
Calculated Molecular Weight 981 aa, 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC146809
Gene Symbol RAB3GAP1
Gene ID (NCBI) 22930
RRIDAB_2882042
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
IHC protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
IF protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

bing (Verified Customer) (08-04-2021)

very good Ab to probe RAB3GAP1 by WB with the right MW of 130 kd

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain and postmortembrain lysates
RAB3GAP1 Antibody Western Blot validation (1:3000 dilution) in mouse brain and postmortembrain lysates (Cat no:66688-1-Ig)