RFWD3 Antibody
Rabbit Polyclonal
Catalog number: 19893-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human testis |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:200-1:1000 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP(RFWD3 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis using 19893-1-AP(RFWD3 Antibody) at Dilution 1:400 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis using 19893-1-AP(RFWD3 Antibody) at Dilution 1:400 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RFWD3 antibody 19893-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | RFWD3 fusion protein ag13728 | Full name | ring finger and WD repeat domain 3 |
|
Calculated molecular weight |
774aa,85kd |
Observed molecular
weight |
85kd |
|
GenBank accession number |
BC002574 | Gene ID (NCBI) | 55159 |
| Gene symbol | RFWD3 | Synonyms | FLJ10520; RNF201 |
Background
RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3. |
