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WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11431-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11431-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11431-2-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11431-2-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11431-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11431-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 11431-2-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 11431-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11431-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11431-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

SESN3 Polyclonal antibody

Name: SESN3 Polyclonal antibody
Brand: Proteintech
SKU: 11431-2-AP

SESN3 Polyclonal Antibody for WB, ELISA

Cat No. 11431-2-AP

Publications(22)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 17 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

11431-2-AP targets SESN3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1961 Product name: Recombinant human SESN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC017296 Sequence: MNRGGGSPSAAANYLLCTNCRKVLRKDKRIRVSQPLTRGPSAFIPEKEVVQANTVDERTNFLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMHVDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDNNIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLFVVSGDTFHSFPHSGAFLHFFAF Predict reactive species
Full Name	sestrin 3
Calculated Molecular Weight	492 aa, 57 kDa
Observed Molecular Weight	36 kDa, 51-59 kDa
GenBank Accession Number	BC017296
Gene Symbol	SESN3
Gene ID (NCBI)	143686
RRID	AB_2185602
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P58005
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Protocols

Product Specific Protocols
WB protocol for SESN3 antibody 11431-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human, mouse	WB,IF	Theranostics Super-enhancer-driven core transcription factor FOXP1 delays endothelial cell senescence via phase separation-mediated SESN3 activation. Authors - Lushuang Mao View Article
human	WB	Dev Cell FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor. Authors - Chia-Chen Chen KD Validated View Article
mouse	IHC	Proc Natl Acad Sci U S A FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis. Authors - Kwang Il Lee View Article
mouse	WB	Cell Death Dis Pifithrin-μ sensitizes mTOR-activated liver cancer to sorafenib treatment Authors - Jiarui Lv View Article
human	WB	Elife Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation. Authors - Veronique Nogueira KD Validated View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner Authors - Sujuan Liu View Article