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WB analysis of HEK-293 using 13657-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13657-1-AP (SLC35A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13657-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13657-1-AP (SLC35A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 13657-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13657-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 13657-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 13657-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 40x lens).

SLC35A2 Polyclonal antibody

Name: SLC35A2 Polyclonal antibody
Brand: Proteintech
SKU: 13657-1-AP

SLC35A2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13657-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SLC35A2, UDP Gal Tr, UDP galactose translocator, UDP galactose transporter, UGALT, UGAT, UGT, UGT1, UGT2, UGTL

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	HEK-293 cells, mouse brain tissue
Positive IHC detected in	human liver cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

13657-1-AP targets SLC35A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4524 Product name: Recombinant human SLC35A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC035747 Sequence: MAAVGAGGSTAAPGPGAVSAGALEPGTASAAHRRLKYISLAVLVVQNASLILSIRYARTLPGDRFFATTAVVMAEVLKGLTCLLLLFAQKRGNVKHLVLFLHEAVLVQYVDTLKLAVPSLIYTLQNNLQYVAISNLPAATFQPSPRCSQSHSLCLCLRLRALRSPAASRAATTTAAVFPPWRPHHGALSAKVSAGEVRAGSNGGTQGRGTGVEGVGHLQDPSRHPPGPGSSGFGRWSFLPGH Predict reactive species
Full Name	solute carrier family 35 (UDP-galactose transporter), member A2
Calculated Molecular Weight	396 aa, 41 kDa
Observed Molecular Weight	41 kDa, 32 kDa
GenBank Accession Number	BC035747
Gene Symbol	SLC35A2
Gene ID (NCBI)	7355
RRID	AB_2877967
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P78381
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for SLC35A2 antibody 13657-1-AP	Download protocol
WB protocol for SLC35A2 antibody 13657-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	IHC,WB	FEBS J SLC35A2 expression drives breast cancer progression via ERK pathway activation Authors - Xiaochen Yang View Article
human	WB	Gene Solute carrier family 35 member A2 regulates mitophagy through the PI3K/AKT/mTOR axis, promoting the proliferation, migration, and invasion of osteosarcoma cells Authors - Xiaohui Luo View Article
	IHC	Front Immunol Comprehensive analysis of 33 human cancers reveals clinical implications and immunotherapeutic value of the solute carrier family 35 member A2 Authors - Shengshan Xu View Article
human	IHC,WB	Aging (Albany NY) Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer Authors - Yue Wang View Article
human	IHC,WB	Cell Oncol (Dordr) Nucleotide sugar transporter SLC35A2 is involved in promoting hepatocellular carcinoma metastasis by regulating cellular glycosylation Authors - Hongxia Cheng KD Validated View Article