Catalog Number..........90013
US + Other Regions Custom Order Form (PDF)(Word) Price - USD............$1388
EU Custom Order Form (PDF)(Word) Price - Euros..........€1388
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Service Description:
Proteintech Group offers fusion protein production starting from the cloning of cDNA into the expression vectors and immunization of two rabbits (Catalog# 90013). Generally, 2 mg of protein will be purified to immunize two rabbits. The customer can choose from one of the standard protocols for immunization. See Catalog#90001 for protocols. The customer will receive the expression cDNA plasmid, 100 ug of fusion protein as a control protein, and antiserum from two rabbits.
The service also includes:
1) Design of the most antigenic and divergent coding region of 300 amino acids or less to serve as the antigen
2) PCR amplification of the desired coding region
3) Subcloning of the cDNA into any expression plasmid that the customer provided
4) Large Scale culture, induction, and purification of the fusion protein.
Guarantee: Proteintech guarantees that upon Proteintech antigen design, the customer will not be charged unless at least 1 mg of fusion protein is successfully expressed and purified as judged by SDS PAGE and western blot. If the customer requested a specific design and the protein did not express enough, a 50% charge will be applied.
Related Services:
Catalog No..........Service
90006................Affinity purification
90003................Cloning and protein expression
90008................Protein expression
90009................Cloning
90136............... .Cloning, protein expression, immunization and affinity purification
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FAQs :
- How do I search for a cDNA on your website?
Go to the home page of our website (www.ptglab.com) and type in any part of the gene description, accession number, or abbreviated gene symbol in the search engine. Click search, then click on the cDNA tab right above the search results. Once an entry is found, please copy the accession number and confirm the sequence in the NCBI website at www.ncbi.nih.gov.
- Which Protocol should I use?
The standard long protocol is the preferred protocol because it has been widely used and tested through history. The long protocol provides a better success rate than the short protocol. The short protocol is designed for customers who need the antibody in a rush and are willing to trade success rate for time.
- How much antiserum will I receive?
Each rabbit will give ~12 ml of serum in each production bleed and 45-50 ml of serum (from 90 - 100 ml blood) in the final bleed, a total of about 150 ml of antiserum from two rabbits.