Catalog Number..........90124
US + Other Regions Custom Order Form (PDF)(Word) Price - USD............$788
EU Custom Order Form (PDF)(Word) Price - Euros..........€788
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Service Description:
Proteintech offers peptide synthesis, conjugation to KLH, and immunization of two rabbits (Catalog# 90124). The conjugated peptide will be used to immunize two rabbits. The customer can choose from one of the standard protocols for immunization. See Catalog#90001 for protocols. The customer will receive the extra unconjugated peptide except for a small amount used for ELISA for the titer assay, and antiserum from two rabbits.
The service also includes the following:
1) Free Peptide Design: Upon customer request, the technical support team of Proteintech will review the protein sequences and rank the most antigenic domains and submit them to the customer for a decision.
2) A large amount and high purity peptide synthesis: The chemists at Proteintech will synthesize the peptide ordered. The yield of synthesis will be approximately 40 mg, at approximately 90% purity for 20 mer-peptides with Mass Spectrum data. There will be no need for additional purification, therefore you do not have to pay for the purification cost, which is a significant savings. We will charge $20 for each additional amino acid.
3) A large amount of KLH conjugation: 10 mg of peptide will be conjugated with 5 mg of KLH protein (Catalog No. 90004).
Guarantee: Proteintech guarantees that the antiserum will recognize the free peptide in either ELISA Assay or Dot Blot Assay.
Related Services:
Catalog No..........Services
90001................Immunization (rabbit polyclonal antibody production)
90002................Peptide synthesis
90004................Peptide conjugation to KLH
90005................Peptide design
90006................Affinity purification
91246.............. ..Peptide design, peptide synthesis, immunization and affinity purification
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FAQs :
- Which kind of antigen should I choose fusion protein or peptide?
Our experience has shown that the fusion protein usually generates an antibody with higher titer and higher specificity. We recommend the fusion protein procedure if a cross reaction can be ignored. However, if you need a specific antibody to a certain protein belonging to one homologous family, we strongly recommend the peptide antibody procedure.
- Which Protocol should I use?
The standard long protocol is the preferred protocol because it has been widely used and tested through history. The long protocol provides a better success rate than the short protocol. The short protocol is designed for customers who need the antibody in a rush and are willing to trade success rate for time.
- How do I order this service?
Download and print out a custom order form, fill out the relevant information, and fax to 312-455-8408 (USA and unlisted regions) or (+44) 161 23 21 272 (Europe). Once we have received your forms someone will contact you to discuss further your order and direct you where to send your protein sample.