Catalog Number..........90136
US + Other Regions Custom Order Form (PDF)(Word) Price - USD............$1999
EU Custom Order Form (PDF)(Word) Price - Euros..........€1999
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Service Description:
Proteintech Group offers fusion protein production starting from the cloning of cDNA into the expression vector that the customer provided, immunization of two rabbits, and affinity purification (Catalog# 90136). Generally, 2 mg of protein will be purified to immunize two rabbits. The customer can choose from one of the standard protocols for immunization. See Catalog#90001 for protocols. Proteintech will prepare an affinity purification column and purify 30ml of antiserum with the column. The customer will receive the expression cDNA plasmid, 100 ug of fusion protein as a control protein, the affinity column, purified anti-serum, flow through, and the rest of the anti-serum from the two rabbits.
The service also includes:
1) Design of the most antigenic and divergent coding region of 300 amino acids or less to serve as the antigen
2) PCR amplification of the desired coding region
3) Subcloning of the cDNA into any expression plasmid that the customer provided
4) Large Scale culture, induction, and purification of the fusion protein.
Guarantee: Proteintech guarantees that upon Proteintech antigen design, the customer will not be charged unless at least 1 mg of fusion protein is successfully expressed and purified as judged by SDS PAGE and western blot. If the customer requested a specific design and the protein did not express enough, a 50% charge will be applied.
Related Services:
Catalog No..........Services
90006................Affinity purification
90003................Cloning and protein expression
90008................Protein expression
90009................Cloning
90013................Cloning, protein expression and immunization
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FAQs :
- How to search for a cDNA on your website?
Go to the home page of our website (www.ptglab.com) and type in any part of the gene description, accession number, or abbreviated gene symbol in the search engine. Click search, then click on the cDNA tab right above the search results. Once an entry is found, please copy the accession number and confirm the sequence in the NCBI website at www.ncbi.nih.gov.
- Which Protocol should I use?
The standard long protocol is the preferred protocol because it has been widely used and tested through history. The long protocol provides a better success rate than the short protocol. The short protocol is designed for customers who need the antibody in a rush and are willing to trade success rate for time.
- How much antiserum will I receive?
Each rabbit will give ~12 ml of serum in each production bleed and 45-50 ml of serum (from 90 - 100 ml blood) in the final bleed, a total of about 150 ml of antiserum from two rabbits.
- How does Proteintech determine which rabbit’s serum to purify?
We decide that by the ELISA result with test bleed. If both rabbits give good results, we will purify 15ml of serum from each rabbit. If one rabbit gives better results than the other, we will purify 30ml of serum from this "good" rabbit.
- How do I order this service?
Download and print out a custom order form, fill out the relevant information, and fax to 312-455-8408 (USA and unlisted regions) or (+44) 161 23 21 272 (Europe). Once we have received your forms someone will contact you to discuss further your order and direct you where to send your protein sample.