Catalog Number..........90186
US + Other Regions Custom Order Form (PDF)(Word) Price - USD............$1699
EU Custom Order Form (PDF)(Word) Price - Euros..........€1699
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Service Description:
Proteintech offers fusion protein production starting from expression vectors provided by the customer, immunization of two rabbits and affinity purification (Catalog# 90186). Fusion protein production includes Large Scale culture, induction, and purification of the fusion protein. Generally, 2 mg of protein will be purified to immunize two rabbits. The customer can choose from one of the standard protocols for immunization. See Catalog#90001 for protocols. Proteintech will prepare an affinity purification column and purify 30ml of antiserum with the column. The customer will receive 100 ug of fusion protein as a control protein, the affinity column, purified anti-serum, flow through, and the rest of the anti-serum from the two rabbits.
Related Services:
Catalog No..........Services
90006................Affinity purification
90003................Cloning and protein expression
90008................Protein expression
90018........... ....Protein expression and immunzation
90013................Cloning, protein expression and immunization
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FAQ :
- What kind of vectors should I provide?
We accept vectors with GST or His Tags. Please tell us the tag, resistance of the vector, molecular weight of the fusion protein, and other related information.
- Which Protocol should I use?
The standard long protocol is the preferred protocol because it has been widely used and tested through history. The long protocol provides a better success rate than the short protocol. The short protocol is designed for customers who need the antibody in a rush and are willing to trade success rate for time.
- How much antiserum will I receive?
Each rabbit will give ~12 ml of serum in each production bleed and 45-50 ml of serum (from 90 - 100 ml blood) in the final bleed, a total of about 150 ml of antiserum from two rabbits
- How does Proteintech determine which rabbit’s serum to purify?
We decide that by the ELISA result with the test bleed. If both rabbits give good results, we will purify 15ml of serum from each rabbit. If one rabbit gives better results than the other, we will purify 30ml of serum from this "good" rabbit.
- How do I order this service?
Download and print out a custom order form, fill out the relevant information, and fax to 312-455-8408 (USA and unlisted regions) or (+44) 161 23 21 272 (Europe). Once we have received your forms someone will contact you to discuss further your order and direct you where to send your protein sample.