Catalog Number..........95012
US + Other Regions Custom Order Form(PDF)(Word) Price - USD............$5299
EU Custom Order Form(PDF)(Word) Price - Euros..........€5299
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Service Description:
Proteintech offers peptide synthesis, conjugation, and monoclonal antibody production (Catalog# 95012). Conjugated peptide will be immunized in 5-10 mice depending on the antigenicity determined by Proteintech. Spleen cells that are harvested will be fused with SP2/0 cells. After culture under selection pressure, the positive clones will be screened against the immunogen. The resulting positive clones will be further subcloned via dilution until stable monoclones are obtained.
The service also includes the following:
1) Free Peptide Design: Upon customer request, the technical support team of Proteintech will review the protein sequences and rank the most antigenic domains and submit them to the customer for a decision.
2) A large amount and high purity peptide synthesis: The chemists at Proteintech will synthesize the peptide ordered. The yield of synthesis will be approximately 40 mg, at approximately 90% purity for 20 mer-peptides with Mass Spectrum data. There will be no need for additional purification, therefore you do not have to pay for the purification cost, which is a significant savings. We will charge $20 for each additional amino acid.
3) A large amount of KLH conjugation: 10 mg of peptide will be conjugated with 5 mg of KLH protein (Catalog No. 90004).
Guarantee: Hybridoma cells, supernantants of culture, and ascites purified by caprylic acid/ammonium sulfate precipitation will be provided.
The success is guaranteed; no positive clone, no charge.
Related services:
Catalog No..........Service
95001.................Mouse monoclonal antibody production
95013.................Cloning, protein expression and monoclonal antibody production
95009.................Mouse ascites production
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FAQs:
- How do I order this service?
Download and print out a custom order form, fill out the relevant information, and fax to 312-455-8408 (USA and unlisted regions) or (+44) 161 23 21 272 (Europe). Once we have received your forms someone will contact you to discuss further your order and direct you where to send your protein sample.