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Willin Polyclonal antibody

Willin Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 21039-1-AP

Synonyms

EX1, FERM domain containing 6, FRMD6, Willin

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using Willin Polyclonal antibody (21039-1-AP)

    WB analysis of U-251 using 21039-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 21039-1-AP (Willin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver cancer using 21039-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver cancer using 21039-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver using 21039-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver using 21039-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of A431 cells using Willin Polyclonal antibody (21039-1-AP)

    FC experiment of A431 using 21039-1-AP

    1X10^6 A431 cells were stained with .2ug Willin antibody (21039-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inU-251 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21039-1-AP is Willin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Willin fusion protein Ag15375
Full Name FERM domain containing 6
Calculated Molecular Weight 622 aa, 72 kDa
GenBank Accession NumberBC029870
Gene Symbol FRMD6
Gene ID (NCBI) 122786
RRIDAB_2878797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Willin, also known as FRMD6 or human Expanded (hEx), is a member of the FERM-domain family of cytoskeletal crosslinkers. It is a potent inhibitor of cell proliferation, transformation and modulator of drug sensitivity, suggesting it possesses tumor suppressor properties. Willin is widely expressed in normal tissues. Although willin is found in the cytoplasm and membrane, it localizes to the nuclei of almost all tumors.This antibody was raised against the C-terminal region of human FRMD6 protein and is expected to recognize the endogenous FRMD6.

Protocols

Product Specific Protocols
WB protocol for Willin antibody 21039-1-APDownload protocol
IHC protocol for Willin antibody 21039-1-APDownload protocol
FC protocol for Willin antibody 21039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols