XIAP Antibody
Rabbit Polyclonal
Catalog number: 10037-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HepG2 cells, COLO 320 cells |
| Positive IHC detected in | human pancreas cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:100-1:500 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HepG2 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig(XIAP Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human pancreas cancer using 10037-1-Ig(XIAP Antibody) at Dilution 1:200 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using XIAP antibody 10037-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Protein A purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | XIAP fusion protein ag0022 | Full name | X-linked inhibitor of apoptosis |
|
Calculated molecular weight |
60kd |
Observed molecular
weight |
45-57kd |
|
GenBank accession number |
BC032729 | Gene ID (NCBI) | 331 |
| Gene symbol | XIAP | Synonyms | API3; BIRC4; ILP1; MIHA; XLP2 |
Background
XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56kd. |
