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ZMYM3 Polyclonal antibody

ZMYM3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 25742-1-AP

Synonyms

DXS6673E, KIAA0385, MYM, XFIM, Zinc finger MYM type protein 3, Zinc finger protein 261, zinc finger, MYM type 3, ZMYM3, ZNF198L2, ZNF261

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ZMYM3 Polyclonal antibody (25742-1-AP)

    WB analysis of mouse brain using 25742-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25742-1-AP (ZMYM3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using ZMYM3 Polyclonal antibody (25742-1-AP)

    IP experiment of K-562 using 25742-1-AP

    IP result of anti-ZMYM3 (IP:25742-1-AP, 4ug; Detection:25742-1-AP 1:300) with K-562 cells lysate 3600ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25742-1-AP is ZMYM3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZMYM3 fusion protein Ag22701
Full Name zinc finger, MYM-type 3
Calculated Molecular Weight 1370 aa, 152 kDa
Observed Molecular Weight 190-200 kDa
GenBank Accession NumberBC013009
Gene Symbol ZMYM3
Gene ID (NCBI) 9203
RRIDAB_2880221
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMYM3, also named as DXS6673E, KIAA0385, and ZNF261, is a 1370 amino acid protein, which may be a component of a BHC histone deacetylase complex. ZMYM3 is most abundant in brain, moderate in muscle and heart, low in other tissues except placenta. ZMYM3 plays a role in the regulation of cell morphology and cytoskeletal organization.

Protocols

Product Specific Protocols
WB protocol for ZMYM3 antibody 25742-1-APDownload protocol
IP protocol for ZMYM3 antibody 25742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols