Phospho-Akt (Tyr315) Antibody
Mouse Monoclonal
Catalog number: 60072-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | NIH/3T3 cells, K-562 cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60072-1-Ig(Phospho-Akt (Tyr315) Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using 60072-1-Ig(Phospho-Akt (Tyr315) Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 60072-1-Ig(Phospho-Akt (Tyr315) Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Phospho Peptide | Full name | v-akt murine thymoma viral oncogene homolog 1 |
|
Calculated molecular weight |
56kd |
Observed molecular
weight |
38+42+66kd |
|
GenBank accession number |
NM_005163 | Gene ID (NCBI) | 207 |
| Gene symbol | AKT1 | Synonyms | AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
Background
Protein kinase B (PKB/Akt) has been well established as an important signaling intermediate, and its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337). Akt, also known as protein kinase B (PKB), is a serine/threonine kinase activated downstream of class I phosphatidylinositol 3-kinase (PI3K) and various receptors. It has emerged as the key regulator for many signal transduction pathways, modulating multiple processes such as cell survival, proliferation, growth, angiogenesis, and glucose metabolism(PMID:16139227). Its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337). This antibody is specific to AKT1. |
