Antibodies are being used in a wide range of biochemical techniques. We listed most of them below but more techniques are being invented on a regular basis.
| Applications |
Abbreviation |
Description |
| Chromatin Immunoprecipitation |
ChIP |
Used to determine the location of DNA binding sites in the genome for a particular protein of interest. |
| Dot Blot |
- |
Used to detect the presence of a substance. |
| Enzyme-Linked ImmunoSorbent Assay |
ELISA |
Detects the presence of a substance in a sample. |
| Flow Cytometry |
FC |
Used to analyze cells or subcellular components by fluorescence emissions. |
| Immunofluorescence |
IF |
Used to localize antigens in cells or in a tissue section by a fluorescent tag. |
| Immunohistochemistry |
IHC |
Used to localize antigens in cells or in a tissue section. |
| Immunoprecipitation |
IP |
Technique used to enrich a protein or protein complex from a mixture. |
| Neutralization |
Neut. |
A neutralizing antibody is capable of binding the antigen and neutralizing the biological effects of that antigen. |
| Radioimmunoassay |
RIA |
A method used to measure the amount of a substance by a radioactive label. |
| Western Blotting |
WB |
Used to detect specific antigens in a sample by applying gel electrophoresis techniques. |
Some of the most widely used antibody applications are described in detail in the following pages.
- ELISA
- Western Blot
- Immunohistochemistry
- Immunofluorescence
- Flow Cytometry
- Immunoprecipitation