ANP32B Polyclonal antibody

• Featured Product
• KD/KO Validated

ANP32B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10843-1-AP

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

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Validation Data Gallery

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  WB analysis of HEK-293 using 10843-1-AP

  WB result of ANP32B antibody (10843-1-AP, 1:5000) with si-control and si-ANP32B transfected HEK293 cells.

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  WB analysis using 10843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10843-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10843-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 10843-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10843-1-AP

  IP result of anti-ANP32B (IP:10843-1-AP, 3ug; Detection:10843-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human colon cancer using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10843-1-AP (ANP32B antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 10843-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ANP32B antibody (10843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, HEK-293T cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below

Product Information

10843-1-AP targets ANP32B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANP32B fusion protein Ag1274
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC013003
• Gene Symbol: ANP32B
• Gene ID (NCBI): 10541
• RRID: AB_2056460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Publications

Species	Application	Title
	WB	Sci Adv; The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals; Authors - Liuke Sun
mouse	WB	Proc Natl Acad Sci U S A; Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development.; Authors - Reilly Patrick T PT
human	WB	Carcinogenesis; Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells.; Authors - Shen Shao-Ming SM ; KD Validated
human	WB	Blood Cancer J; PMID: 24413065; Authors - B C Valdez
human	WB,IHC	Cell Death Dis; ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.; Authors - S Yang ; KD Validated
human	WB	J Virol; ANP32 proteins are essential for influenza virus replication in human cells.; Authors - Ecco Staller ; KO Validated