SODD/BAG4 Polyclonal antibody

SODD/BAG4 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13913-1-AP

Synonyms

BAG 4, BAG4, Silencer of death domains, SODD

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Validation Data Gallery

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  WB analysis of K-562 using 13913-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13913-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13913-1-AP

  IP result of anti-SODD/BAG4 (IP:13913-1-AP, 4ug; Detection:13913-1-AP 1:500) with K-562 cells lysate 2800ug.

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  IF Staining of MCF-7 using 13913-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IP detected in: K-562 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13913-1-AP targets SODD/BAG4 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: Artemia sinica
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SODD/BAG4 fusion protein Ag4905
• Full Name: BCL2-associated athanogene 4
• Calculated Molecular Weight: 457 aa, 50 kDa
• Observed Molecular Weight: 60 kDa, 66-70 kDa
• GenBank Accession Number: BC038505
• Gene Symbol: BAG4
• Gene ID (NCBI): 9530
• RRID: AB_2243308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).

Publications

Species	Application	Title
Artemia sinica	WB	Gene; Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.; Authors - Mengchen Zhang
	WB	Nat Commun; Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila; Authors - Alexandra E. Perlegos