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BMP7 Polyclonal antibody

BMP7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12221-1-AP

Synonyms

BMP 7, BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human kidney tissue, human renal cell carcinoma tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12221-1-AP targets BMP7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMP7 fusion protein Ag2902
Full Name bone morphogenetic protein 7
Calculated Molecular Weight 431 aa, 49 kDa
Observed Molecular Weight 43-49 kDa
GenBank Accession NumberBC008584
Gene Symbol BMP7
Gene ID (NCBI) 655
RRIDAB_2063960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

Protocols

Product Specific Protocols
WB protocol for BMP7 antibody 12221-1-APDownload protocol
IHC protocol for BMP7 antibody 12221-1-APDownload protocol
IF protocol for BMP7 antibody 12221-1-APDownload protocol
IP protocol for BMP7 antibody 12221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Circ Res

Yin Yang 1 Suppresses Dilated Cardiomyopathy and Cardiac Fibrosis Through Regulation of Bmp7 and Ctgf.

Authors - Chia Yee Tan
WB,IF

Cell Mol Life Sci

Omentin-1 drives cardiomyocyte cell cycle arrest and metabolic maturation by interacting with BMP7

Authors - Huijun Yang
ratWB

Nanoscale

Concentrated small extracellular vesicles from menstrual blood-derived stromal cells improve intrauterine adhesion, a pre-clinical study in a rat model.

Authors - Siwen Zhang
WB

Front Mol Neurosci

Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.

Authors - Tianda Chen
ratWB,IHC,IF

Front Pharmacol

BMP-7 Upregulates Id2 Through the MAPK Signaling Pathway to Improve Diabetic Tubulointerstitial Fibrosis and the Intervention of Oxymatrine.

Authors - Yawen Xiao
WB

BMC Complement Med Ther

Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway

Authors - Ying Chen