Bcl-XL Polyclonal antibody

• Featured Product
• KD/KO Validated

Bcl-XL Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Publications

171

Reviews (2)

Cat no : 10783-1-AP

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL

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Validation Data Gallery

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  WB analysis using 10783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 10783-1-AP

  WB result of Bcl-XL antibody (10783-1-AP, 1:800) with si-Control and si-Bcl-XL transfected HeLa cells.

• 

  WB analysis of Raji using 10783-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10783-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10783-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10783-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10783-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 10783-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10783-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10783-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10783-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of k-562 using 10783-1-AP

  IP result of anti-Bcl-XL (IP:10783-1-AP, 4ug; Detection:10783-1-AP 1:1000) with k-562 cells lysate 1760 ug.

• 

  IHC staining of human lung cancer using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 10783-1-AP

  Immunofluorescent analysis of () fixed NIH/3T3 cells using 10783-1-AP (Bcl-XL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of Jurkat using 10783-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Bcl-XL (10783-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: A549 cells, Raji cells, Jurkat cells, rat brain tissue, HeLa cells, NIH/3T3 cells, human placenta tissue, C6 cells, K-562 cells, mouse placenta tissue, RAW264.7
• Positive IP detected in: k-562 cells
• Positive IHC detected in: human lung cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 159 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

10783-1-AP targets Bcl-XL in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat, sheep, zebrafish, hamster, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Bcl-XL fusion protein Ag1232
• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: BCL2L1
• Gene ID (NCBI): 598
• RRID: AB_2064724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta) , and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Publications

Species	Application	Title
		Immunity; cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity; Authors - Yunlong Zhao
human	WB	Nat Commun; Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction.; Authors - Tian-Ying Zhang
human	WB	Carbohydr Polym; A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer; Authors - Shaofeng Duan
human,mouse	WB	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
rat	WB	Oxid Med Cell Longev; Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.; Authors - Jiazheng Chen
rat	WB	Oxid Med Cell Longev; Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway.; Authors - Bo Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In cancer cell lines, the bands appeared significantly clearer, and the optimal concentration was found to be 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lung cancer cell lines

Bcl-XL Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:10783-1-AP)

FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0,05µl/10^6 cells
• Cell Tissue Type: B cells

Bcl-XL Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:10783-1-AP)

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