Caspase 1/p20/p10 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 1/p20/p10 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

665

Reviews (2)

Cat no : 22915-1-AP

Synonyms

CASP 1, CASP1, Caspase 1, Caspase 1/p20/p10, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, p10, p20, P45

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Validation Data Gallery

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  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22915-1-AP

  WB result of Caspase 1/p20/p10 antibody (22915-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 1/p20/p10 transfected HepG2 cells.

• 

  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22915-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 22915-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (CASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22915-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (CASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22915-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (CASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 22915-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (CASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22915-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (CASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 22915-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 22915-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of C6 using 22915-1-AP

  Immunofluorescent analysis of C6 cells using 22915-1-AP (Caspase 1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, HeLa cells, C6 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells, THP-1 cells, PC-12 cells, U-937 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 603 publications below
• IHC: See 111 publications below
• IF: See 115 publications below
• IP: See 1 publications below

Product Information

22915-1-AP targets Caspase 1/p20/p10 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, pig, canine, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 1/p20/p10 fusion protein Ag11143
• Full Name: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Calculated Molecular Weight: 404 aa, 45 kDa
• Observed Molecular Weight: 45-47 kDa, 30 kDa, 35 kDa
• GenBank Accession Number: BC062327
• Gene Symbol: CASP1
• Gene ID (NCBI): 834
• RRID: AB_2876874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20.

Publications

Species	Application	Title
mouse	WB	Mol Cancer; Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases; Authors - Qiang Wang
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
mouse	WB,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
mouse	WB	Nat Commun; REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.; Authors - Shuai Wang
mouse	WB	Theranostics; Ablation of NLRP3 inflammasome attenuates muscle atrophy via inhibiting pyroptosis, proteolysis and apoptosis following denervation; Authors - Zongqi You
mouse	WB	J Clin Invest; Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.; Authors - Abhishek Jauhari

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joseph (Verified Customer) (02-19-2020)

This antibody detected pro-caspase 1 and the p20 cleaved product in PDAC cell lines. I did not look for the p10 cleaved product.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PDAC cell lines

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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