CBFA2T2 Polyclonal antibody

CBFA2T2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, chIP,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11336-1-AP

Synonyms

CBFA2T2, EHT, MTG8 like protein, MTG8 related protein 1, MTGR1, p85, Protein CBFA2T2, ZMYND3

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Validation Data Gallery

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  WB analysis of HEK-293 using 11336-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11336-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11336-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11336-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11336-1-AP (CBFA2T2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse testis tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• ChIP: See 1 publications below

Product Information

11336-1-AP targets CBFA2T2 in WB, IHC, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBFA2T2 fusion protein Ag1880
• Full Name: core-binding factor, runt domain, alpha subunit 2; translocated to, 2
• Calculated Molecular Weight: 604 aa, 67 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC015066
• Gene Symbol: CBFA2T2
• Gene ID (NCBI): 9139
• RRID: AB_2070571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Core-binding factor, runt domain, alpha subunit 2, translocated to 2(CBFA2T2) belongs to a family of ubiquitously expressed transcriptional repressors that interact with transcription factors bound to promoters of target genes and with histone deacetylases (HDACs) critical for enforcement of repressor function.CBFA2T2, also named as MTGR1 or EHT, is an 85-kDa phosphoprotein. It can bind AML1-MTG8 fusion protein in acute myeloid leukemia. The binding leads to an enhancement of cell proliferation mediated in a murine myeloid model. The calculated molecular weight of CBFA2T2 is 67 kDa, but modified SMARCE1 is about 80 kDa.

Publications

Species	Application	Title
	chIP	Oncogene; Myeloid translocation genes differentially regulate colorectal cancer programs.; Authors - B Parang