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  • KD/KO Validated

CD320 Polyclonal antibody

CD320 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10343-1-AP

Synonyms

8D6, 8D6 antigen, 8D6A, CD320, CD320 antigen, CD320 molecule, FDC signaling molecule 8D6, FDC SM 8D6, TCblR, Transcobalamin receptor



Tested Applications

Positive WB detected inHL-60 cells, human liver tissue, HEK-293 cells, mouse heart tissue, HeLa cells, Raji cells, human placenta tissue
Positive IHC detected inmouse kidney tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10343-1-AP targets CD320 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, cat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD320 fusion protein Ag0344
Full Name CD320 molecule
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 60-70 kDa, 58 kDa
GenBank Accession NumberBC000668
Gene Symbol CD320
Gene ID (NCBI) 51293
RRIDAB_2074223
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 58 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

Protocols

Product Specific Protocols
WB protocol for CD320 antibody 10343-1-APDownload protocol
IHC protocol for CD320 antibody 10343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canine, catIHC

Oncotarget

Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.

Authors - Annette M Sysel
mouseIF

Int J Biol Macromol

CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.

Authors - Yue Chen
humanWB,IF

FASEB J

Identification of a novel mechanism for meso-tetra (4-carboxyphenyl) porphyrin (TCPP) uptake in cancer cells.

Authors - David J Elzi
  • KD Validated
humanIHC

Anticancer Res

Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.

Authors - Sysel Annette M AM
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen
humanIF

Cell Rep

FTY720 requires vitamin B12-TCN2-CD320 signaling in astrocytes to reduce disease in an animal model of multiple sclerosis

Authors - Deepa Jonnalagadda

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joseph (Verified Customer) (09-17-2020)

We evaluated several CD320 antibodies but the Proteintech sequence provided better specificity and greater sensitivity to the CD320 protein target.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline
  • Cell Tissue Type: human, canine and feline malignant various