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CHRNA7 Monoclonal antibody

CHRNA7 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

2H1G3

Cat no : 60220-1-Ig

Synonyms

CHRNA7, CHRNA7 2, NACHRA7

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CHRNA7 Monoclonal antibody (60220-1-Ig)

    WB analysis of MCF-7 using 60220-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 60220-1-Ig (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHRNA7 Monoclonal antibody (60220-1-Ig)

    IHC staining of human brain using 60220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60220-1-Ig (CHRNA7 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CHRNA7 Monoclonal antibody (60220-1-Ig)

    IHC staining of human brain using 60220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60220-1-Ig (CHRNA7 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inMCF7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 60220-1-Ig is CHRNA7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CHRNA7 fusion protein Ag15980
Full Name cholinergic receptor, nicotinic, alpha 7
Calculated Molecular Weight 502 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC037571
Gene Symbol CHRNA7
Gene ID (NCBI) 1139
RRIDAB_11043180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin.

Protocols

Product Specific Protocols
WB protocol for CHRNA7 antibody 60220-1-IgDownload protocol
IHC protocol for CHRNA7 antibody 60220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Functional Human α7 Nicotinic Acetylcholine Receptor (nAChR) Generated from E. coli.

Authors - Tommy S Tillman
View Article