DH5a-lysate Polyclonal antibody

DH5a-lysate Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

bacteria

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10559-1-Ig

Synonyms

DH5a lysate, DH5a-lysate

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Validation Data Gallery

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  WB analysis using 10559-1-Ig

  Total DH5a lysates were subjected to SDS PAGE followed by western blot with 10559-1-Ig (DH5a-lysate antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Total bacteria lysates of DH5 alpha

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10559-1-Ig targets DH5a-lysate in WB, ELISA applications and shows reactivity with bacteria samples.

• Tested Reactivity: bacteria
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Bacterial Lysates
• Full Name: DH5a-lysate
• Calculated Molecular Weight: 1-300 kDa
• RRID: AB_1607766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is generated with the total bacteria lysate of DH5a. This antibody can be used to detect the host cell protein (HCP) residual.