FHL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FHL1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 10991-1-AP

Synonyms

FHL 1, FHL1, FHL1B, FLH1A, four and a half LIM domains 1, KYO T, Skeletal muscle LIM protein 1, SLIM, SLIM 1, SLIM1, XMPMA

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Validation Data Gallery

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  WB analysis using 10991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10991-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10991-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 10991-1-AP

  IP result of anti-FHL1 (IP:10991-1-AP, 3ug; Detection:10991-1-AP 1:700) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human ovary tumor using 10991-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10991-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human brain tissue, mouse colon tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 23 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Product Information

10991-1-AP targets FHL1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.

• Tested Reactivity: human, mouse, zebrafish, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FHL1 fusion protein Ag1448
• Full Name: four and a half LIM domains 1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010998
• Gene Symbol: FHL1
• Gene ID (NCBI): 2273
• RRID: AB_2104230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Four-and-a-half LIM domains 1 (FHL1), a member of the FHL protein family, which composed of FHL 1, 2, 3, 4 and ACT (activator of CREM), and characterized by an N-terminal half LIM domain followed by four complete LIM domains. FHL1 may contribute to sarcomere synthesis, assembly and biomechanical stress sensing. FHL1 is also a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, which contributes to the formation of an epigenetic field for cancerization. FHL1(10991-1-AP) is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. This is a rabbit polyantibody raised against full length of human FHL1.

Publications

Species	Application	Title
human	WB,IHC,CoIP,ChIP	J Clin Invest; Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.; Authors - Ding Lihua L ; KD Validated
human	WB	Genes Dev; Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.; Authors - Anne Loft ; KD Validated
human	WB,IHC	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu ; KD Validated
		J Cell Biol; Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter.; Authors - Xiang Wang
human	WB,IHC	Int J Cancer; Downregulation and growth inhibitory role of FHL1 in lung cancer.; Authors - Niu Chang C
human,mouse	WB	J Cell Sci; MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.; Authors - Leitner Laura L