Human CCL5 ELISA Kit

Sensitivity

0.3 pg/mL

Range

3.9 - 250 pg/mL

Reactivity

Human

Cat no : KE00093

Synonyms

C C motif chemokine 5, CCL5, chemokine (C C motif) ligand 5, D17S136E, EoCP, RANTES, SCYA5, SIS delta, SISd, Small inducible cytokine A5, T cell specific protein P228, TCP228



Product Information

KE00093 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CCL5 ELISA kit is to be used to detect and quantify protein levels of endogenous human CCL5. The assay recognizes human CCL5. An antibody specific for human CCL5 has been pre-coated onto the microwells. The human CCL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL5 is added to detect the captured human CCL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human CCL5 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 0.3 pg/mL
Range 3.9 - 250 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 6352

Recovery

Sample TypeAverageRange
Urine86%69%-107%
Human plasma94%80%-111%
Cell culture supernatants105%85%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12024.51.25.1
22060.72.13.5
320227.519.08.3

InterAssay

Samplenmean ( pg/mL)SDCV%
12424.61.87.2
22461.14.06.6
324236.018.67.9

Background Information

CCL5, also known as RANTES, belongs to the CC chemokine family. It is strongly expressed in the human and murine liver upon injury. CCL5 has been found to be produced by numerous cell types, including platelets, macrophages, endothelial, and stellate cells. CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells, and basophils to sites of inflammation by interacting with 3 specific G protein-coupled receptors: CCR1, CCR3, and CCR5. While CCL5 may promote efficient anti-tumour immune responses, it has also been associated with cancer progression and metastasis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms C C motif chemokine 5, CCL5, chemokine (C C motif) ligand 5, D17S136E, EoCP, RANTES, SCYA5, SIS delta, SISd, Small inducible cytokine A5, T cell specific protein P228, TCP228

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Adv Sci (Weinh)

S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma

Authors - Huihuang Li
humancell culture supernatants

JCI Insight

Mutant RB1 enhances therapeutic efficacy of PARPis in lung adenocarcinoma by triggering the cGAS/STING pathway

Authors - Qi Dong
humancell culture supernatants

Aging (Albany NY)

CCL5-dependent mast cell infiltration into the tumor microenvironment in clear cell renal cell carcinoma patients.

Authors - Tianjie Liu
humanserum

J Immunol

Macrophagic Extracellular Vesicle CXCL2 Recruits and Activates the Neutrophil CXCR2/PKC/NOX4 Axis in Sepsis.

Authors - Guozhen Wang
humancell culture supernatants

Regen Biomater

Concentrated growth factor regulates the macrophage-mediated immune response.

Authors - Haiyun Luo
humancell culture supernatants

Adv Sci (Weinh)

BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity

Authors - Zhiyong Cai