MACC1 Polyclonal antibody

MACC1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21970-1-AP

Synonyms

7A5, MACC1, SH3BP4L

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Validation Data Gallery

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  IHC staining of human liver cancer using 21970-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 21970-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 21970-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21970-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 21970-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 21970-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21970-1-AP targets MACC1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MACC1 fusion protein Ag16884
• Full Name: metastasis associated in colon cancer 1
• Calculated Molecular Weight: 852 aa, 97 kDa
• GenBank Accession Number: BC137090
• Gene Symbol: MACC1
• Gene ID (NCBI): 346389
• RRID: AB_2878957
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MACC1, Metalloantigenolytic Cleavage Cartilage Proteinase 1, belongs to the ADAM (a disintegrin and metalloprotease) family. MACC1 was first identified in colon cancer. It is a key prognostic biomarker and is involved in recurrence, metastasis, and survival in solid cancers (PMID: 23815185). MACC1 expression is primarily observed in cartilage, but it's also present in other tissues like bone, synovium, and the developing nervous system (PMID: 36979146).

Publications

Species	Application	Title
		Mol Carcinog; The role of metastasis-associated in colon cancer 1 (MACC1) in endometrial carcinoma tumorigenesis and progression.; Authors - Shuo Chen