MPZ / P0 Polyclonal antibody

MPZ / P0 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10572-1-AP

Synonyms

CHM, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, DSS, HMSNIB, MPP, MPZ, MPZ / P0, Myelin peripheral protein, Myelin protein P0, myelin protein zero, P0

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Validation Data Gallery

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  WB analysis of Schwann cells using 10572-1-AP

  WB result from Chattopadhyay S (PMID:19229995); Schwann cell differentiation was induced with dbcAMP (500 μM) for 48 h, followed by treatment with rhMMP-9 (100 nM) for 15 min. A, rhMMP-9 stimulates transient ERK1/2 activation over a 1 h period. Successful Schwann cell differentiation was confirmed by myelin protein zero (P0) expression, β-actin was used as loading control.

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  IF Staining of myelinating SCs cells using 10572-1-AP

  IF result from Huaqing Liu (PMID:20448483); Dual immunofluorescence for BrdU (red) with myelinating Schwann cell markers P0 (10572-1-AP, green, 1:150) in distal segments of MMPi-treated nerves. .

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Tested Applications

• Positive WB detected in: Schwann cells
• Positive IF detected in: myelinating SCs cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

10572-1-AP targets MPZ / P0 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, frog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPZ / P0 fusion protein Ag0848
• Full Name: myelin protein zero
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC006491
• Gene Symbol: MPZ
• Gene ID (NCBI): 4359
• RRID: AB_2144657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPZ (myelin protein zero), also known as P0, is a transmembrane glycoprotein that belongs to the immunoglobulin supergene family. Synthesized by myelin-forming Schwann cells, MPZ is the major structural protein component of myelin in the peripheral nervous system. It is involved in the formation and maintenance of compact myelin, and plays a role in the creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. More than 120 mutations detected in the gene of MPZ cause various forms of hereditary neuropathy, which include Charcot-Marie-Tooth disease type 1B (CMT1B), CMT2, Dejerine-Sottas syndrome (DSS), and congenital hypomyelination neuropathy (CHN). This antibody can recognize endogenous MPZ and can be used as a marker of myelinating Schwann cells. MPZ can be detected the 25-30 kDa band by western blot. A band of 36 kDa could also be detected, which is a novel isoform of MPZ that contains an additional domain at the C-terminal (PMID: 22457349). It can also exist as a dimer (PMID: 12933931).

Publications

Species	Application	Title
rat	WB	Oxid Med Cell Longev; Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2.; Authors - Chunyang Xu
rat	WB	Front Cell Neurosci; Protein kinase C epsilon activation regulates proliferation, migration, and epithelial to mesenchymal-like transition in rat Schwann cells; Authors - Tasnim Mohamed
human	WB	Front Endocrinol (Lausanne); Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension; Authors - He Ma
rat	IF	Stem Cell Res Ther; Schwann cells promote prevascularization and osteogenesis of tissue-engineered bone via bone marrow mesenchymal stem cell-derived endothelial cells.; Authors - Xinxin Zhang
rat	WB	Glia; MMP-9 controls Schwann cell proliferation and phenotypic remodeling via IGF-1 and ErbB receptor-mediated activation of MEK/ERK pathway.; Authors - Chattopadhyay Sharmila S
mouse	WB,IF	Mol Cell Biol; DHTKD1 deficiency causes Charcot-Marie-Tooth disease in mice.; Authors - Wang-Yang Xu