MYOZ1 Polyclonal antibody

MYOZ1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13160-1-AP

Synonyms

Calsarcin 2, CS 2, FATZ, MYOZ, MYOZ1, myozenin 1, Protein FATZ

---

Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 13160-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13160-1-AP (MYOZ1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of human heart using 13160-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13160-1-AP (MYOZ1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 13160-1-AP

  IP result of anti-MYOZ1 (IP:13160-1-AP, 4ug; Detection:13160-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human heart tissue
• Positive IP detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

13160-1-AP targets MYOZ1 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYOZ1 fusion protein Ag3806
• Full Name: myozenin 1
• Calculated Molecular Weight: 299 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC025753
• Gene Symbol: MYOZ1
• Gene ID (NCBI): 58529
• RRID: AB_2282208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myozenin-1 is a protein that in humans is encoded by the MYOZ1 gene. MYOZ1 has been shown to interact with Telethonin, and Actinin, alpha. The MW of this protein is 32 kDa, and Catalog#13160-1-AP specially recognises the 32 kDa protein.

Publications

Species	Application	Title
rat	WB	Front Physiol; NO-Dependent Mechanisms of Myosin Heavy Chain Transcription Regulation in Rat Soleus Muscle After 7-Days Hindlimb Unloading.; Authors - Kristina A Sharlo
fish	WB	J Muscle Res Cell Motil; Reduced expression of MyHC slow isoform in rat soleus during unloading is accompanied by alterations of endogenous inhibitors of calcineurin/NFAT signaling pathway.; Authors - Yulia N Lomonosova
rat	WB	J Appl Physiol (1985); Plantar mechanical stimulation prevents calcineurin/NFATc1 inactivation and slow-to-fast fiber-type shift in rat soleus muscle under hindlimb unloading.; Authors - Kristina Sharlo